Tissue Bath Type 813/6 Perfusion of Tubular Organs

• Perfusion of tubular organs (trachea, blood vessels, intestines)

• Individual solutions for intraluminal perfusions and extraliminal superfusion

• Controlled perfusion pressure

This Tissue Bath has been designed for the studying of perfused tubular organs such as trachea, blood vessels, intestines and vas deferens. Individual solutions can be used for intraluminal perfusion and extraluminal superfusion. Two roller pumps are needed, one for intraluminal constant flow perfusion and the second for extraluminal superfusion. The effective intraluminal perfusion is generated by an adjustable afterload control system. A differential pressure transducer is used to measure the intraluminal pressure difference at the proximal and distal end of the organ. The tissue bath is a water-jacketed Plexiglas bath. The holder for the cannulae can be removed from the main bath during the cannulation of the segment of tubular organs. Four different sizes of cannulae are available. The cannulae are fixed on sliding holders to optimize the distance between the cannulae to adapt to the individual length of the organ avoiding any slackness of the tissue. There are two afterload control systems available. One for low pressure applications (0 to 20 mmHg) and the second for high pressure applications (10 to 300 mmHg). The unit is supplied with four different interchangeable adapters for use with vessels or organs with the following diameters: 1.5, 2.5, 3 and 4 mm. For smaller vessels customized stainless steel cannulae can be made on request. For micro-vessels, glass capillary pipettes pulled to the required diameter can be connected to the adapter cannulae using a silicone tube collar. Maximum vessel length is 50 mm.