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公司名称： |
美国自然基因有限公司 |
| 发布日期： |
2008-1-17 |
| 所 在 地： |
美国自然基因有限公司 |
| 已获点击： |
316次 |
| 简单介绍： |
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| 用于许多体内,离体，卵内电穿孔基因转移，包括药物及基因的传递 |
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btx genetrodes是一种成对针式可重复使用的电极。它是体内，卵内电穿孔，包括药物基因转移的理想选择。genetrodes有五种型号可供选择，以适应各种电穿孔领域的不同尺寸形状要求。每种型号包括一对金质电极针，有直头和l型两种。在注入感兴趣的分子后，将电极放入组织中，用btx电穿孔系统施加电脉冲，所产生的电场会使组织细胞上出现短暂的通道，允许分子摄入胞内。电极可以用电极夹持器在组织内以一种预设的间隙平行放置。
btx genetrodes are needle-style reusable electrodes that are used for a variety of applications. genetrodes come in five models, with each model consisting of a pair of electrodes. the model 508 and 510 genetrodes are straight electrodes with gold tips. the model 512, 514, and 516 genetrodes are bent l-shaped electrodes with gold tips. these electrodes are connected to a model 465 square-post connection cable and interfaced with a pulse generator via a model 5343 coaxial-banana connection cable. the genetrodes are positioned in parallel at a predetermined gap within a tissue using the model 515 genetrode holder. genetrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. properly maintained genetrodes have a life span of approximately 1500+ pulses, and are compatible with most btx electroporation systems.
applications
general applications: genetrodes may be used for many in vivo and in ovo electroporation applications, including drug and gene delivery. the electrodes are placed into a tissue following injection of the molecule of interest, and an electroporation pulse is delivered using a btx electroporation system, such as the ecm 600, 630, 830, t820 or 2001. the electric field introduced by the genetrodes causes transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells.
in vivo applications: model 508 and model 510 genetrodes: the model 508 genetrode with a sharp 5mm electrode tip is a general in vivo electrode. the model 510 is the same electrode but with a 10mm tip. genetrodes have been used for a variety of applications. eide et al. used the genetrodes along with the ecm 830 to introduce plasmid dna into free swimming embryonic xenopus laevis.2 dean et al. used the genetrodes on mouse cornea, which resulted in a 30-fold higher expression than that obtained with lipofectin.3 murphy et al. used the genetrodes with ecm 830 to introduce plasmid dna into a mouse cerebellum, where he concluded that electroporation is a viable means of transferring genes to the central nervous system.4 aihara and miyazaki used these electrodes with a t820 to transfer genes into murine muscle tissue, significantly increasing expression levels over dna injection alone.5
in ovo applications: model 512, 514, and 516 genetrodes: the model 512, 514, and 516 electrodes are all suitable for in ovo gene transfer studies. kos used the genetrodes with the t820 to successfully introduce expressing plasmids and morpholino antisense oligonucleotides in ovo 1 and paquette et al. used the genetrodes to drive plasmid dna into the neural tube of a developing egg in which widespread gene expression was observed.9 an electrode similar to model 514 has been used with a t820 by muramatsu in his in ovo studies.5 he concludes that electroporation is superior to both lipofection and microparticle bombardment.6
technical specifications
standard capabilities
voltage range: 0 - 200 vdc
pulse length range: 1µs - 99msec
physical characteristics
in vivo genetrodes model 508 model 510
electrode length: 5mm 10mm
shaft configuration: long long
in vivo genetrode tips are invasive, gold coated and 0.5mm in diameter. the shafts are straight and 0.8mm in diameter.
in ovo genetrodes model 512 model 514 model 516
electrode length: 5mm 3mm 1mm
in ovo genetrode tips are non-invasive, gold coated and 0.5mm in diameter. the shafts are 0.8mm in diameter and l-shaped.
generator compatibility ecm® 630, 830, 2001, 600, & t820
genetrode holder interelectrode gaps 1-10mm
ordering information model desc-ription part number
508 in vivo straight electrode w/ 5mm gold tip 10-001849-01
510 in vivo straight electrode w/ 10mm gold tip 10-001849-02
512 in ovo l-shaped electrode w/ 5mm gold tip 10-001850-01
514 in ovo l-shaped electrode w/ 3mm gold tip 10-001850-02
516 in ovo l-shaped electrode w/ 1mm gold tip 10-002509-01
515 genetrode holder 10-002560-01
465 square-post connection cable 06-700044-01
5343 coaxial-banana connection cable 06-700042-01
references
kos et al., development, 128: 1467-1479 (2001)
eide et al., febs letters, 486: 29-32 (2000)
dean et al., the journal of gene medicine, 4: 92-100 (2002)
murphy and messer, molecular therapy, 3 (1): 113-121 (2001)
aihara and miyazaki, nature biotechnology, 16: 867-870 (1998)
personal communication, ryuya tanaka, creative laboratories, osaka, japan (1998)
muramatsu, mizutani, and okumura, anim. sci. technol. (jpn.) 67 (10): 906-909 (1996)
muramatsu et al., biochemical and biophysical research communications, 230: 376-380 (1997)
paquette et al., pnas, 97: 12318-12323 (2000) |
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